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张翠军课题组

张翠军课题组

Zhang Cuijun Lab

  课题组长

张翠军,研究员,博导。2010年硕博连读毕业于河北师范大学,其后相继在北京生命科学研究所、上海植物逆境生物学研究中心、美国普渡大学开展研究,2019年加入农业基因组所。其课题组利用基因组学、遗传学、生化与分子生物学等多学科手段开展小麦抗赤霉病及基因编辑改良研究。重点研究: 1) 禾谷镰刀菌与小麦互作;2) 小麦抗赤霉病机理及新基因挖掘;3)小麦基因编辑改良。在 Nature Communications, The EMBO Journal, Cell Research, Plant Biotechnology Journal, J Integr Plant Biol等国际一流期刊上发表多篇论文。

联系方式:zhangcuijun@caas.cn。


  工作经历

  中国农业科学院农业基因组研究所        研究员            2019.10–至今

  中国科学院植物研究所                        研究员             2019.5–2019.9

  普渡大学                                            博士后             2015.8–2019.4

  上海植物逆境生物学研究中心              副研究员          2015.4–2015.8

  北京生命科学研究所                           研究助理          2010.7–2015.4

 

  教育经历

  河北师范大学                            博士&硕士           2005.9–2010.6

  河北师范大学                            本科                    2001.9–2005.6

 

  团队研究方向

1.小麦赤霉病是小麦最严重的病害之一,主要由禾谷镰刀菌 (Fusarium graminearum) 引起,每年给小麦生产造成巨大损失,并导致小麦籽粒毒素污染,对人类和牲畜健康构成严重威胁。近几年气候变化和耕作制度改变,赤霉病的发生具有愈来愈严重的趋势。我们联合应用多种技术对小麦赤霉病发病机制及小麦抗赤霉病机理开展深入研究,并对特殊小麦品系赤霉病抗性进行评价,筛选小麦赤霉病新抗源,克隆新的关键抗病基因,为小麦抗赤霉病改良提供基础;

2.普通小麦为异源六倍体,复杂的基因组限制了小麦功能基因的发现?;虮嗉际踉诙啾短遄魑锕δ芑蚍⑾旨耙糯牧挤矫婢哂芯薮笥攀疲颐怯τ没虮嗉际醵孕÷蠡肪呈视π约翱剐越懈咝Ц牧肌?/span>

 

  研究进展

1.通过质谱对禾谷镰刀菌的分泌蛋白组进行了研究,发现了多个能够引起植物细胞死亡的分泌性蛋白,其中发现五个枯草杆菌蛋白酶(Subtilisin-like family proteases, FgSLPs)作为毒素蛋白能够引物植物非特异性细胞死亡,是禾谷镰刀菌致病关键蛋白。

2.通过研究小麦与禾谷镰刀菌互作,发现了多个禾谷镰刀菌致病性效应子蛋白及其小麦中的靶蛋白,揭示了禾谷镰刀菌致病性效应子蛋白介导的新型致病机制。

3.通过 CRISPR/Cas9 基因编辑技术修复Tamyb10-B1a基因的移码突变,使Tamyb10-B1基因恢复功能,提高了小麦穗发芽抗性。


发表论文

1. Shengping Shang; Yuhan He; Qianyong Hu; Ying Fang; Shifeng Cheng; Cui-Jun Zhang* (2024). Fusarium graminearum effector FgEC1 targets wheat TaGF14b protein to suppress TaRBOHD‐mediated ROS production and promote infection. J Integr Plant Biol., doi.org/10.1111/jipb.13752

2. Xishi Zhou, Yujin Fan, Xiying Zhu, Ruihua Zhao, Junna He, Pengfeng Li, Shengping Shang, Justin Goodrich, Jian-Kang Zhu, Cui-Jun Zhang* (2024). SANT proteins modulate gene expression by coordinating histone H3KAc and Khib levels and regulate plant heat tolerance. Plant Physiology, doi.org/10.1093/plphys/kiae348.

3. Jiang Xiong, Mingyu Luo, Yunshen Chen, Qianyong Hu, Ying Fang, Tongjun Sun, Guanjing Hu, Cui-Jun Zhang* (2024). Subtilisin-like proteases from Fusarium graminearum induce plant cell death and contribute to virulence. Plant Physiology, 195, 1681–1693.

4. Yiwang Zhu#, Yarong Lin#, Yujin Fan, Yiwei Wang, Pengfeng Li, Jiang Xiong, Yuhan He, Shifeng Cheng, Xingguo Ye, Feng Wang, Justin Goodrich, Jian-Kang Zhu, Ke Wang*, and Cui-Jun Zhang* (2023). CRISPR/Cas9-mediated restoration of Tamyb10 to create pre-harvest sprouting-resistant red wheat. Plant Biotechnology Journal, 21, 665–667.

5. Lina Zhang#, Xishi Zhou#, Pengfeng Li, Yiwei Wang, Qianyong Hu, Yuping Shang, Yunshen Chen, Xiying Zhu, Hongjie Feng*, and Cui-Jun Zhang* (2023). Transcriptome Profile of Fusarium graminearum Treated by Putrescine. J. Fungi, 9, 60.

6. Yarong Lin#, Yiwang Zhu#, Yuchao Cui#, Hongge Qian#, Qiaoling Yuan#, Rui Chen, Yan Lin, Jianmin Chen, Xishi Zhou, Chuanlin Shi, Huiying He, Taijiao Hu, Chenbo Gu, Xiaoman Yu, Xiying Zhu, Yuexing Wang, Qian Qian, Cui-Jun Zhang*, Feng Wang*, and Lianguang Shang* (2023). Identification of natural allelic variation in TTL1 controlling thermotolerance and grain size by a rice super pan‐genome. J Integr Plant Biol., 65: 2541–2551.

7. Xishi Zhou#, Junna He#, Christos N. Velanis#, Yiwang Zhu, Yuhan He, Kai Tang, Mingku Zhu, Lisa Graser, Erica de Leau, Xingang Wang, Lingrui Zhang, W. Andy Tao, Justin Goodrich*, Jian-Kang Zhu*, Cui-Jun Zhang* (2021). A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis. J Integr Plant Biol., 63, 1462–1474.

8. Cuijun Zhang#, Xuan Du#, Kai Tang#, Zhenlin Yang, Li Pan, Peipei Zhu, Jinyan Luo, Yuwei Jiang,Hui Zhang, Huafang Wan, Xingang Wang, Fengkai Wu, W. Andy Tao, Xin-Jian He, Heng Zhang, Ray A. Bressan, Jiamu Du*, Jian-Kang Zhu* (2018). Arabidopsis AGDP1 links H3K9me2 to DNA methylation in heterochromatin. Nature Communications, 9, 4547.

9. Lian-Mei Tan#, Cui-Jun Zhang#, Xiao-Mei Hou, Chang-Rong Shao, Yu-Jia Lu, Jin-Xing Zhou, Yong-Qiang Li, Lin Li, She Chen, Xin-Jian He* (2018). The PEAT protein complexes are required for histone deacetylation and heterochromatin silencing. EMBO Journal, 37, e100573.

10. Cui-Jun Zhang#, Xiao-Mei Hou#, Lian-Mei Tan, Chang-Rong Shao, Huan-Wei Huang, Yong-Qiang Li, Lin Li, Tao Cai, She Chen, Xin-Jian He* (2016). The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing. Nature Communications, 7, 11715.

11. Cui-Jun Zhang#, Jin-Xing Zhou#, Jun Liu#, Ze-Yang Ma, Su-Wei Zhang, Kun Dou, Huan-Wei Huang, Tao Cai, Renyi Liu, Jian-Kang Zhu, Xin-Jian He* (2013). The splicing machinery promotes RNA-directed DNA methylation and transcriptional silencing in Arabidopsis. EMBO Journal, 32, 1128-40.

12. Cui-Jun Zhang, Yong-Qiang Ning, Su-Wei Zhang, Qing Chen, Chang-Rong Shao, Yan-Wu Guo, Jin-Xing Zhou, Lin Li, She Chen, Xin-Jian He* (2012). IDN2 and its paralogs form a complex required for RNA-directed DNA methylation. PLoS Genetics, 8, e1002693.

13. Jun Liu#, Ge Bai#, Cuijun Zhang#, Wei Chen, Jinxing Zhou, Suwei Zhang, Qing Chen, Xin Deng, Xin-Jian He*, Jian-Kang Zhu (2011). An atypical component of RNA-directed DNA methylation machinery has both DNA methylation-dependent and-independent roles in locus-specific transcriptional gene silencing. Cell Research, 21, 1691-1700.

14. Cuijun Zhang, and Yi Guo* (2012). OsTRXh1 regulates the redox state of the apoplast and influences stress responses in rice. Plant Signal Behav., 7, 441-443.

15. Cui-Jun Zhang#, Bing-Chun Zhao#, Wei-Na Ge, Ya-Fang Zhang, Yun Song, Da-Ye Sun, and Yi Guo* (2011). An apoplastic h-type thioredoxin is involved in the stress response through regulation of the apoplastic reactive oxygen species in rice. Plant Physiology, 157, 1884-1899.

16. Yun Song#, Cuijun Zhang#, Weina Ge, Yafang Zhang, Alma L. Burlingame, Yi Guo* (2011). Identification of NaCl stress-responsive apoplastic proteins in rice shoot stems by 2D-DIGE. J Proteomics, 74, 1045-1067.


  张翠军课题组更新于2024年8月

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