汪泉课题组

　　Wang Quan Lab

　　首席科学家/课题组长介绍：

　　汪泉，研究员，博士生导师。主要研究领域为植物发育生物学。研究成果在Plant Cell、New Phytologist和Plant Journal等著名学术期刊发表。

　　Quan Wang, Principal Investigator.  Dr Wang’s research focuses on plant development biology. And he has published several papers on Plant Cell, New Phytologist, Plant Journal and so on.

　　

　　研究成果：

　　论著：

　　1. Wang Q, Rossmann S, Hasson A and Theres K (2015): Divide et impera: boundaries shape the plant body and initiate new meristems. New Phytologist. DOI: 10.1111/nph.13641;

　　2. Wang Q, Kohlen W, Rossmann S, Vernoux T and Theres K (2014): Auxin Depletion from the Leaf Axil Conditions Competence for Axillary Meristem Formation in Arabidopsis and Tomato. Plant Cell. 26(5):2068-2079

　　3. Yang F#, Wang Q#, Schmitz G, Müller D and Theres K (2012) The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis. Plant Journal. 71:61-70 #同等贡献

　　4. Shi B*, Zhang C*, Tian C, Wang J, Wang Q, Xu T, Xu Y, Ohno C, Sablowski R, Heisler M G, Theres K, Wang Y and Jiao Y. (2016) Two-step regulation of a meristematic cell population acting in shoot branching in Arabidopsis. PLoS Genet. 12: e1006168

　　

　　团队研究方向: 

　　叶是植物重要的器官，是植物光合作用的场所，是重要的碳源，热源，并能将氧气和水份回馈到大气中。团队利用功能基因组学和细胞生物学等手段研究水稻叶极性建成发育的分子机制。通过分子遗传学，组织学，活体成像，转录组学分析等手段挖掘水稻叶极性建成的关键调控基因，解析水稻叶极性建成的遗传调控网络，揭示水稻叶极性建成的分子机制。通过对叶极性发育的研究，不但可以加深植物形态建成的理论认识，还能够为育种提供重要的遗传资源。

　　Leaf is an important plant organ, it is the place for plant photo-synthesis. And leaf is also the heat source, carbon source and it can feed oxygen, water to atmosphere. Our lab studies the mechanism of leaf development, especially leaf polarity establishment in rice. Through different methods, like histology, live imaging and transcriptome analysis, we would like to find out key genes for leaf polarity establishment and characterize function of these genes.  And we try to build up a genetic regulation network and understand molecular mechanism. Through the study of leaf development, it can shed more light on plant morphogenesis theory and also provide important genetic resources for breeding

　　

　　研究进展：

　　叶从植物顶端分生组织起始，随着生长，叶会形成三个极性：近-远轴，背-腹轴，中-边轴。水稻叶的平展性依赖于这三个极性的建成，近-远轴决定叶片和叶鞘的分化，背-腹轴决定叶片的卷曲度，而中-边轴决定叶的宽窄。

　　团队前期筛选获得一株明显狭窄的叶发育缺陷突变体(narrow leaf1)，图位克隆和互补验证表明窄叶表形是由于生长素合成相关基因OsTAR1（FISHBONE）突变缺陷引起的。该结果表明，生长素和叶片的中-边轴极性建成相关。而生长素和细胞分裂素通常在植物发育中是相互拮抗的，后续我们将围绕生长素和细胞分裂素对水稻叶中-边轴极性建成展开系统研究。

　　Leaves are initiated from the flank of shoot apical meristem. As they grow, three axes are established: Proximal-distal axis, Adaxial-abaxial axis, Medio-Lateral axis. Rice leaf flatten process depends on these axes: Proximal-distal axis determines leaf sheath and leaf blade development, adaxial-abaxial axis regulates leaf rolling level, and medio-lateral axis modulates leaf width.

　　We identified a leaf defect mutant and named narrow leaf1, which has significantly narrower leaf compared to wild type (Figure 1). Map-based cloning and complementation experiments suggested that narrow leaf defects was caused by a mutation of an auxin biosynthesis gene OsTAR1/FISHBONE, indicating that auxin plays an important role in rice leaf medio-lateral axis establishment. As auxin and cytokinin usually perform the function antagonistically, we will try to investigate the function of both hormones in rice leaf medio-lateral axis establishment.  　

 

汪泉课题组更新于2020年7月